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Merck
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主要文件

SAB5700611

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

rabbit polyclonal

别名:

ALXDRD, GFAP

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100 μL
$597.00

$597.00

预计发货时间2025年8月04日详情

新价格，新优惠！
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100 μL
$597.00

About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

$597.00

预计发货时间2025年8月04日详情

新价格，新优惠！

产品名称

Anti-GFAP antibody produced in rabbit,

生物来源

rabbit

质量水平

100

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

49
 50

种属反应性

human, mouse, rat

浓度

2.32 mg/ml

技术

immunofluorescence: 1:50-1:200
western blot: 1:500-1:2000

UniProt登记号

P14136

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... GFAP(2670)

一般描述

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

免疫原

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).

外形

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

储存及稳定性

Store at -20℃. Avoid freeze / thaw cycles.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
3560492113
3560492111

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Questions

  1. こんにちは、マウスのアストロサイトにおけるGFAP含有量を測定したいと考えています。免疫蛍光法を使用する予定で、現在Anti-GFAP （SAB5700611）とAnti-Rabbit IgG (H+L)（SAB4600045）の2つの抗体を選んでいます。これらの抗体は理論的には相互に結合できますか？ また、これら2つの抗体の推奨濃度についても教えていただけると嬉しいです。 よろしくお願いいたします。

    1 answer

    1. Anti-GFAP (SAB5700611) is a primary antibody targeting the Glial fibrillary acidic protein found in astrocytes. When used in conjunction with Anti-Rabbit IgG (H+L) (SAB4600045) in an experiment, e.g., immunofluorescence or western blot, the goat-produced Anti-Rabbit IgG (H+L) secondary antibody (SAB4600045) would bind to the rabbit-produced Anti-GFAP primary antibody. This is because the secondary antibody is specific for rabbit immunoglobulins, and the primary antibody is produced in rabbits, thus containing rabbit IgG.
      Regarding the working concentrations, see the dilutions factors for each item below. Please note that this information is also listed in the Properties section of the product page.
      SAB5700611
      immunofluorescence: 1:50-1:200
      western blot: 1:500-1:2000

      SAB4600045
      flow cytometry: 1-10 μg/mL
      immunocytochemistry: suitable
      immunohistochemistry: suitable
      indirect immunofluorescence: 1-10 μg/mL

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