SAB4200571
Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal
clone NSE-P1, purified from hybridoma cell culture
别名:
Anti-2-phospho-D-glycerate hydrolyase, Anti-Enolase 2 (gamma, neuronal), Anti-NSE, Anti-Neural enolase, Anti-gamma-enolase, Monoclonal Anti-2-phospho-D-glycerate hydro-lyase
登录查看公司和协议定价
所有图片(4)
About This Item
分類程式碼代碼:
12352203
NACRES:
NA.41
推荐产品
生物源
mouse
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
NSE-P1, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~47 kDa
物種活性
human, rat, mouse
濃度
~1.0 mg/mL
技術
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.
western blot: 0.5-1.0 μg/mL using NTERA-2 (NT2/D1) total cell extracts.
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ENO2(2026)
一般說明
Monoclonal anti-neuron-specific enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Neuron specific enolase (NSE) belongs to the family of enolase enzymes. Enolases have three subunits (a, b, and g), which can combine to form five different isoenzymes: aa, ab, ag, bb, and gg. Enolase 1 (aa) is present in adipose tissue, kidney, liver, and spleen. Enolase 3 (bb) is muscle specific. Enolase 2 (ENO2), also known as neuron-specific enolase (NSE) is a cytoplasmic enzyme, that is released during cell destruction and has a half-life of approximately 30 hours in serum. This dimeric enzyme (gg) is found in neurons and in neuroendocrine cells. ENO2 gene is mapped to human chromosome 12p13.
特異性
Monoclonal Anti- Neuron-Specific Enolase (NSE) recognizes human, rat, and mouse NSE.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal region of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
應用
Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal has been used in:
- immunofluorescence staining
- immunocytochemistry
- immunofluorescence microscopy
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunocytochemistry (1 paper)
生化／生理作用
Enolase 2 (ENO2) participates in glycolysis by converting β-glycerophosphate into dihydroxyacetone phosphate. It serves as a neurobiochemical marker of brain damage after traumatic brain injury, anoxic encephalopathy, stroke, and cardiac arrest. NSE also acts as a circulating marker for neuroendocrine tumors. High levels of NSE is observed in breast cancer upon exposure to arsenite and cadmium.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
儲存和穩定性
For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Stanislav Rodkin et al.
International journal of molecular sciences, 24(21) (2023-11-14)
Traumatic brain injury (TBI) is one of the leading causes of disability and death worldwide. It is characterized by various molecular-cellular events, with the main ones being apoptosis and damage to axons. To date, there are no clinically effective neuroprotective
Martha Douglas-Escobar et al.
Frontiers in neurology, 3, 144-144 (2012-11-07)
As neonatal intensive care has evolved, the focus has shifted from improving mortality alone to an effort to improve both mortality and morbidity. The most frequent source of neonatal brain injury occurs as a result of hypoxic-ischemic injury. Hypoxic-ischemic injury
Cheng-Cheng Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 46(4), 1525-1535 (2018-04-25)
The metabolic features of cancer cells have long been acknowledged to be altered and to provide new therapeutic opportunities. The expression of glycolytic enzyme enolase 2 (ENO2) was found to be closely associated with the clinical features of acute lymphoblastic
Malin Rundgren et al.
BMC research notes, 7, 726-726 (2014-10-17)
Neuron specific enolase (NSE) is a recognized biomarker for assessment of neurological outcome after cardiac arrest, but its reliability has been questioned. Our aim was to investigate what influence storage of samples and choice of measuring methods may have on
S P Craig et al.
Cytogenetics and cell genetics, 54(1-2), 71-73 (1990-01-01)
We have localised the human cDNA for neurone-specific enolase (ENO2) to chromosome region 12p13 by in situ hybridisation. Two additional smaller peaks of hybridisation to specific chromosomal subregions were observed. That on chromosome 1p36 probably represents cross-hybridisation to the locus
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门