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Merck

S6402

Sigma-Aldrich

链霉亲和素 −Cy3 来源于阿维丁链霉菌

buffered aqueous solution

别名:

Streptavidin Cy3

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

共軛

CY3 conjugate

形狀

buffered aqueous solution

標籤範圍

3-9 mol fluorochrome per mol protein

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

链霉亲和素是一种同源四聚体蛋白。一分子链亲和素通过非共价相互作用与四分子生物素结合——这种相互作用基本上是不可逆的。链霉亲和素对生物素具有高亲和力,广泛用作标记工具及涉及结合蛋白的研究。

應用

阿维迪尼链霉菌链霉亲和素 −Cy3 已用于
  • :双重免疫荧光染色检测生物素凝集素
  • 免疫荧光分析中的胰岛素和 C 肽染色
  • 双重免疫荧光,以验证是否存在充满生物胞素的细胞
  • 与生物素化抗体联合应用于免疫组化和免疫细胞化学
  • 由于光谱重叠,与传统的罗丹明滤光片联用

特點和優勢

Cy3 染料的光谱特性:
  • 吸收最大值 552nm
  • 发射最大值 565nm
  • 摩尔消光系数 1.3 x 10-5 M-1cm-1

外觀

0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液,含 1% 牛血清白蛋白和 15 mM 叠氮化钠

法律資訊

Cy3 is a trademark of Cytiva

免責聲明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、 体外 诊断用途、 离体 或 体内 治疗用途或任何类型的消费或人类或动物应用。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Hiroyuki Nobusue et al.
Cell and tissue research, 332(3), 435-446 (2008-04-04)
We established a preadipocyte cell line from mature adipocytes obtained from subcutaneous fat tissue of green fluorescent protein (GFP) transgenic mice. The floating top layer, containing mature adipocytes, was isolated from subcutaneous fat tissue by collagenase digestion and filtration. Fluorescence-activated
Ji-Myung Bae et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(6), 1126-1140 (2018-02-07)
The Sp7/Osterix transcription factor is essential for bone development. Mutations of the Sp7 gene in humans are associated with craniofacial anomalies and osteogenesis imperfecta. However, the role of Sp7 in embryonic tooth development remains unknown. Here we identified the functional
Agnieszka E Gorska et al.
The American journal of pathology, 163(4), 1539-1549 (2003-09-26)
We have previously shown that expression of a dominant-negative type II transforming growth factor-beta receptor (DNIIR) in mammary epithelium under control of the MMTV promoter/enhancer causes alveolar hyperplasia and differentiation in virgin mice. Here we show that MMTV-DNIIR female mice
Niklas Schwarz et al.
eLife, 8 (2019-09-10)
Most of our knowledge on human CNS circuitry and related disorders originates from model organisms. How well such data translate to the human CNS remains largely to be determined. Human brain slice cultures derived from neurosurgical resections may offer novel
Miroslav Hons et al.
Nature immunology, 19(6), 606-616 (2018-05-20)
Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together

商品

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

可用的荧光原位杂交(FISH)过程、试剂和设备。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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