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Merck

S6147

Sigma-Aldrich

葡聚糖凝胶 ® G-100

BioReagent, for molecular biology, DNA grade, medium

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.52

等級

DNA grade
for molecular biology

品質等級

產品線

BioReagent

形狀

beads

技術

buffer exchange: suitable

基質活性組

phase

膨潤

1 g swells to 9-11 mL

珠直徑

40-120 μm

孔徑

exclusion limit (25 bp dsDNA; (rA)27)

pH值

2-10

應用

life science and biopharma

相容性

Cytiva

異物活動

DNase, RNase, none detected

儲存溫度

room temp

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一般說明

Sephadex® G-100葡聚糖凝胶(中级)是经常被文献引用的凝胶过滤基质,主要用于超大分子的脱盐和缓冲液置换。 Sephadex®葡聚糖凝胶由葡聚糖(dextran)与环氧氯丙烷交联而成。Sephadex产品有各种不同的交联度,因此其溶胀程度和分子分离范围也不同。 “中级”代表较大颗粒,主要用于要求高流速和低操作压力的大规模组群分离。

應用

Sephadex ® G-100 适用于离心柱纯化 DNA(来自污染的小分子),用于杂交。采用凝胶过滤法分离纯化香蕉中多酚氧化酶。

特點和優勢

  • 极佳的回收率
  • 最小样品稀释度
  • 快速且易于使用

法律資訊

Sephadex is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Dae-Young Lee et al.
Journal of medicinal food, 15(4), 399-405 (2012-02-10)
Grains of sugary rice were extracted with 80% aqueous methanol, and the concentrated extracts were successively partitioned using ethyl acetate, n-butanol, and water. From the n-butanol fractions, four flavonoid glycosides were isolated through repeated silica gel, octadecyl silica gel, and
Lila Boulekbache-Makhlouf et al.
Phytochemical analysis : PCA, 24(2), 162-170 (2012-08-30)
Eucalyptus species are widely cultivated in Mediterranean regions. Moreover, plants of this family have been utilized for medicinal purposes. A number of studies have been devoted to the identification of eucalypt phenolics, all of them have focused on specific families

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