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Merck

S1629

Sigma-Aldrich

硫酸脂酶 来源于产气杆菌

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

别名:

芳基硫酸酯酶, 苯硫酸酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type VI

品質等級

形狀

buffered aqueous glycerol solution

比活性

2-5 units/mg protein (biuret)

分子量

~41 kDa

濃度

10-20 units/mL

異物活動

β-Glucuronidase ≤10 U/mL

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

硫酸酯酶包含Cys/Ser-X-Pro-X-Arg基序,保存在它们的活性部位。

應用

产氧菌磺化酶已用于:
  • 用作解凝酶处理血浆样品,通过液相色谱串联质谱(LC/MS/MS)分析进行槲皮素定量
  • 基于荧光强度的酶活性检测,使用基于活性的探针1
  • 处理硫硫醇脂质体,以去除硫脂中的3-O-硫代半乳糖苷头

生化/生理作用

硫酸酯酶水解硫酸酯键生成无机硫酸盐。微生物硫化酶参与硫的清除,是硫利用的必需酶。它们可能与发病机制有关。来自产气杆菌的市售硫酸盐酶可用作解凝酶,用于从缀合物中去除葡萄糖醛酸盐和硫酸盐部分。硫酸酯酶广泛用于工业和农业。

單位定義

在pH 7.1,37℃条件下,一个单位可在每分钟水解1.0 μmole硝基苯磷酸盐。

外觀

溶于含0.01 M Tris的50%甘油,pH 7.5。

基底

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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T Saidha et al.
Archives of biochemistry and biophysics, 272(1), 237-244 (1989-07-01)
Mitochondria that have been purified from cells of light-grown wild-type Euglena gracilis Klebs var. bacillaris Cori or dark-grown mutant W10BSmL and incubated with 35SO4(2-) and ATP accumulate a labeled compound in the surrounding medium. This compound is also labeled when
Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Toyokazu Miura et al.
Bioscience, biotechnology, and biochemistry, 70(6), 1509-1512 (2006-06-24)
Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme
Bioluminescent probes of sulfatase activity.
Jason S Rush et al.
Chembiochem : a European journal of chemical biology, 11(15), 2096-2099 (2010-09-28)

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