The capture antibody utilized in this kit is a mouse monoclonal antibody with an immunogen of aa32-194 of human FGF-7 (accession # P21781). Meanwhile, the detection antibody is a goat polyclonal antibody with the immunogen of aa32-194 of human FGF-7 (accession # Q6FGV5).
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物種活性
human
包裝
kit of 96 wells (12 strips x 8 wells)
技術
ELISA: suitable
capture ELISA: suitable
輸入
sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.55-400 pg/mL
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... FGF7(2252)
一般說明
The Human FGF-7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF-7 in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Human FGF7
應用
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
生化／生理作用
FGF7 (fibroblast growth factor 7) is involved in the development of skin and sweat glands, wound healing and cutaneous homeostasis.[4] It controls keratinocyte differentiation and viability by modulating autophagy.[2] FGF7 also participates in embryonic development, tumorigenesis, cell proliferation, migration and differentiation.[3] It is linked with several disorders like nonsyndromic cleft lip and palate, thyroid volume, goiter risk and chronic obstructive pulmonary disease. FGF7 plays an important role in the morphology of developing lungs. Absence of the FGF7 activity decreases airway branching during development. During injury, it is involved in airway repair.[5]
其他說明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
訊號詞
Warning
危險聲明
防範說明
危險分類
Met. Corr. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
Association between fibroblast growth factor 7 and the risk of chronic obstructive pulmonary disease
Xu SC, et al.
Acta Pharmacologica Sinica, 33(8), 998-1003 (2012)
Recombinant keratinocyte growth factor 1 in tobacco potentially promotes wound healing in diabetic rats
Feng ZG, et al.
BioMed Research International (2014)
FGF7 Over Expression is an Independent Prognosticator in Patients with Urothelial Carcinoma of the Upper Urinary Tract and Bladder
Fan EW, et al.
The Journal of Urology, 194(1), 223-229 (2015)
Oncogenic mutations of ALK kinase in neuroblastoma
Chen Y, et al.
Nature, 455(7215), 971-974 (2008)
Role of Keratinocyte Growth Factor in the Differentiation of Sweat Gland-Like Cells From Human Umbilical Cord-Derived Mesenchymal Stem Cells.
Xu Y
Stem Cells Translational Medicine, 5(1), 106-116 (2016)
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What is the specificity of the Human FGF7 ELISA antibodies (RAB0188)?
1 answer-
Helpful?
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Will the Human FGF-7 ELISA kit (BAB0188) detect mouse FGF7?
1 answer-
The Human FGF-7 ELISA kit has not been tested for cross-reactivity to mouse, so we cannot confirm if it can detect mouse FGF-7. However, we do offer a Mouse FGF-7 ELISA with the catalog number RAB1123.
Helpful?
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Is there a detailed protocol for this (the general sandwich protocol is not specific enough)? E.g. the kit FGF-7 concentrate for the standard curve - no concentration is written on the vial. And there is no information on how to make serial dilutions.
1 answer-
Using the lot number on the product you purchased, you can access the lot-specific CofA to view the procedure and concentration. Example CofA using lot number 0922J0245: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0188_0922J0245.pdf
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