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一般說明
麦胚 tRNA 含有许多不同的特异性 tRNA 分子。
應用
粗转移 RNA (tRNA) 从小麦胚芽中提纯,含有许多特定的 tRNA 分子,可以通过不同的过程分离。利用 体外 翻译系统进行详细的结构和功能研究实验通常需要高纯度的 tRNA,这可以通过色谱分离和 tRNA 的可逆生化修饰的组合来实现。麦胚 tRNA 已用于从麦胚纯化 RNAse Z(tRNA 3′-处理核酸内切酶)。纯化包括 6 个步骤,其中最有效的步骤是使用由麦胚 tRNA 构成的亲和柱,其与 RNAse Z 紧密结合。
适合作为核酸纯化和沉淀中的载体使用。
成分
tRNA 以冻干粉形式提供,可溶于水,浓度为 2 mg/mL。
單位定義
在 1.0 mL 水中(1 cm 光径),1 个单位在 260 nm 处的吸光度为 1.0。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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