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形狀
buffered aqueous glycerol solution
濃度
10,000 units/mL
運輸包裝
wet ice
儲存溫度
−20°C
特異性
Recognition sequence: 5′-C/C(A,T)(T,A)GG-3′
Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
其他說明
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
外觀
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1% gelatine (v/v), 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
K Mise et al.
Gene, 33(3), 357-361 (1985-01-01)
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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