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Merck

R4526

Sigma-Aldrich

定量PCR参考染料

100 ×, solution

别名:

qPCR参考染料, ROX

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

solution

品質等級

用途

sufficient for ≥600 reactions

濃度

100 ×

技術

PCR: suitable

顏色

red

溶解度

water: soluble

儲存溫度

2-8°C

一般說明

Sigma′定量聚合酶链反应(qPCR)参考染料是一种专有染料,用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时,它可以用于反应数据的标准化。 我们提供100×参考染料,最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。

應用

定量 PCR 参考染料已被用于:
  • 制备用于实时定量聚合酶链反应 (RT-qPCR)的预混液
  • 作为反应混合物的成分,用于通过定量聚合酶链式反应(qPCR)检测艰难梭菌
  • 通过 qPCR 分析细胞 DNA 污染程度

其他說明

定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。

象形圖

Exclamation markEnvironment

訊號詞

Warning

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Gustav Johansson et al.
Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
The majority of patients diagnosed with advanced gastrointestinal stromal tumors (GISTs) are successfully treated with a combination of surgery and tyrosine kinase inhibitors (TKIs). However, it remains challenging to monitor treatment efficacy and identify relapse early. Here, we utilized a
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)

商品

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

实验方案

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

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