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生物来源
bakers yeast
表单
lyophilized powder
比活
50-100 units/mg protein (modified Warburg-Christian)
异质活性
phosphoriboisomerase, alcohol dehydrogenase, transketolase, and transaldolase <0.1%
储存温度
−20°C
应用
D-Ribulose-5-phosphate 3-Epimerase is an enzyme that converts the reversible conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate, which is important for the cellular response against oxidative stress [1]. D-Ribulose-5-phosphate 3-Epimerase is involved in the pentose phosphate pathway, pentose and glucuronate interconversions and carbon fixation. Product R3251 is from baker′s yeast and is provided as a lyophilized powder. It is useful in enzyme systems requiring low sulfate.
生化/生理作用
RPE is a metalloenzyme and has been shown to use the divalent Zn2+ ion predominantly for catalysis. Human D-ribulose-5-phosphate 3-epimerase (hRPE) has been shown to use Fe2+ for catalysis [1].
单位定义
在 pH 7.7 和 25°C 下，与转酮醇酶、α-甘油磷酸脱氢酶和磷酸丙糖异构酶偶联时，一单位每分钟可将 1μmol 的 D-核酮糖-5-磷酸转化为 D-木酮糖-5-磷酸。
外形
基本上无硫酸盐的冻干粉：约含35% 的柠檬酸缓冲盐
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Helena Sävenstrand et al.
Plant & cell physiology, 43(4), 402-410 (2002-04-30)
Suppression subtractive hybridisation was used to isolate genes differentially regulated by low levels (UV-B(BE,300) 0.13 W m(-2)) of ultraviolet-B radiation (UV-B; 290-320 nm) in Pisum sativum. Six genes were regulated, two of which were novel. The mRNA levels for these
Matthew B Rogers et al.
BMC evolutionary biology, 7, 89-89 (2007-06-15)
Lateral gene transfer is increasingly invoked to explain phylogenetic results that conflict with our understanding of organismal relationships. In eukaryotes, the most common observation interpreted in this way is the appearance of a bacterial gene (one that is not clearly
Kui K Chan et al.
Biochemistry, 47(36), 9608-9617 (2008-08-15)
Enzymes that share the (beta/alpha) 8-barrel fold catalyze a diverse range of reactions. Many utilize phosphorylated substrates and share a conserved C-terminal (beta/alpha) 2-quarter barrel subdomain that provides a binding motif for the dianionic phosphate group. We recently reported functional
J Caruthers et al.
Proteins, 62(2), 338-342 (2005-11-24)
The crystal structure of Pfal009167AAA, a putative ribulose 5-phosphate 3-epimerase (PfalRPE) from Plasmodium falciparum, has been determined to 2 A resolution. RPE represents an exciting potential drug target for developing antimalarials because it is involved in the shikimate and the
M Teige et al.
FEBS letters, 377(3), 349-352 (1995-12-27)
A cDNA clone encoding the chloroplast enzyme pentose-5-phosphate 3-epimerase (EC 5.1.3.1) in potato (Solanum tuberosum) was isolated and sequenced. The deduced sequence of 235 amino acids is similar to protein sequences of bacterial epimerases. Northern blot analysis showed the highest
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