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Merck

P9614

Sigma-Aldrich

λ 蛋白磷酸酶

liquid, Bacteriophage Lambda, recombinant, expressed in E. coli

别名:

λ 酶

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

形狀

liquid

分子量

25 kDa

濃度

≥400000 units/mL

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

λ 蛋白磷酸酶是一种在 大肠杆菌中表达的重组蛋白 。它需要 Mn 2 + 或 Ni 2 + 作为活化剂。

生化/生理作用

λ 蛋白磷酸酶已被用于对海拉细胞提取物进行去磷酸化。
可用于从蛋白质中的丝氨酸、苏氨酸或酪氨酸残基释放磷酸基团。对磷酸化组氨酸残基也有活性。

單位定義

在 30℃,pH 7.5的条件下,一单位每分钟水解 1 nmol 对硝基苯磷酸盐。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
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The Journal of Biological Chemistry, 268(24), 17754-17761 (1993)
Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.
Ishii T
The Journal of Biological Chemistry, 285(53), 41993-42000 (2010)
P T Cohen et al.
The Biochemical journal, 260(3), 931-934 (1989-06-15)
Infection of Escherichia coli with phage lambda gt10 resulted in the appearance of a protein phosphatase with activity towards 32P-labelled casein. Activity reached a maximum near the point of cell lysis and declined thereafter. The phosphatase was stimulated 30-fold by
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