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生物源
human
品質等級
重組細胞
expressed in E. coli
化驗
≥98% (HPLC and SDS-PAGE)
形狀
lyophilized powder
效力
1.0-3.0 ng/mL EC50
品質
endotoxin tested
分子量
~25 kDa
包裝
pkg of 10 μg
pkg of 50 μg
儲存條件
avoid repeated freeze/thaw cycles
技術
cell culture | mammalian: suitable
雜質
≤1 EU/μg
UniProt登錄號
儲存溫度
−20°C
基因資訊
human ... PDGFB(5155)
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一般說明
Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.
應用
Platelet-Derived Growth Factor-BB human has been used :
- As a growth supplement in proximal tubule and glomerular explant cell culture
- In three-dimensional scaffold preparation
- To study the effect of brivanib on growth factor signaling pathways
- In transwell migration assay
生化／生理作用
Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
其他說明
Dimer of the B chain of human PDGF.
外觀
Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.
分析報告
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis.
Kohannim O,et al.
Case Reports in Genetics, 2011 , 1-6 (2011)
Matrix stiffness regulates migration of human lung fibroblasts.
Asano1 S, et al.
Physiological Reports, 5.9, 1-11 (2017)
Islet grafting and imaging in a bioengineered intramuscular space
Witkowski P, et al.
Transplantation, 88.9, 1065-1065 (2009)
Yasutoshi Takashima et al.
American journal of physiology. Renal physiology, 310(5), F395-F408 (2016-01-15)
Glomerular integrity and functions are maintained by growth factor signaling. Heparan sulfate, the major component of glomerular extracellular matrixes, modulates growth factor signaling, but its roles in glomerular homeostasis are unknown. We investigated the roles of heparan sulfate 6-O-endosulfatases, sulfatase
Injury induces in vivo expression of platelet-derived growth factor (PDGF) and PDGF receptor mRNAs in skin epithelial cells and PDGF mRNA in connective tissue fibroblasts
Antoniades HN
Proceedings of the National Academy of Sciences of the USA, 88.2, 565-569 (1991)
商品
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
血小板衍生生长因子是在哺乳动物血清中发现的主要有丝分裂原，在血栓形成过程中从血小板释放。
实验方案
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
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