In most circumstannces, the product is used at a 100-fold dilution. This will inhibit the phosphatases in 5 mg of protein per mL of lysate.
推荐产品
一般說明
磷酸酶抑制剂混合液 1 包括将抑制碱性磷酸酶的 L -同工酶以及丝氨酸/苏氨酸蛋白磷酸酶（如 PP1 和 PP2A）的抑制剂混合物。本品含微囊藻毒素LR、斑蝥素和（−）-p-溴四唑。磷酸酶抑制剂混合液1 已被替换为 P0044。
生化／生理作用
磷酸酶是许多重要生物过程的活性调节剂。小分子对磷酸酶的抑制有助于研究其生物学作用。[4]
數量
推荐的用法是 100 倍稀释。
分析報告
检测牛肝脏、人胎盘和兔肌肉等动物组织的细胞提取物，以及 A431 和 Jurkat 细胞系的提取物。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
188.6 °F
閃點（°C）
87 °C
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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商品
磷酸酶抑制剂对于所有类型的磷酸化研究都至关重要。对比磷酸酶抑制剂混合物，选择更适合您的研究的产品。
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
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At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How stable is Product P2850, Phosphatase Inhibitor Cocktail 1? Can I freeze it?
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This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.
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Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?
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As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.
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What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?
1 answer-
Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A). Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.
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Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?
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Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C). Simply allow it to melt before use. This will not affect the product.
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