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Merck
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文件

P2069

Sigma-Aldrich

苯酚:氯仿:异戊醇 25:24:1，10 mM Tris（pH 8.0）、1 mM EDTA饱和

Supplied with Equilibration buffer, for molecular biology

别名:

DNA extraction

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.52

等級

for molecular biology

化驗

≥99.0%

形狀

liquid

pH值

6.5-6.9(pH of phenolic phase)
7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

應用

agriculture

儲存溫度

2-8°C

應用

对于需要更高 pH 值的应用，例如大的完整基因组 DNA的分离，建议添加平衡缓冲液。
苯酚：氯仿：异戊醇已用于制备 RNA，用于研究从心肌活检中分离总 RNA。它还被用于在人雄激素受体（HUMARA） 克隆性检测中提取 DNA。

包裝

随配 P3803 和一瓶单独的平衡缓冲液，用于将苯酚相调节至 pH 8.0 ± 0.2。

適合性

这批苯酚：氯仿：异戊醇 25:24:1 采用从大肠杆菌中提取 的pBR322 DNA 的粗制品进行了测试。 根据琼脂糖凝胶电泳评估，提取的 DNA 没有降解或浓度降低。 采用相同的操作程序处理纯化的 pBR322 DNA 和 λ Hind III 消化 DNA。 根据琼脂糖凝胶电泳评估，纯化的制剂没有检测到降解或浓度降低。

準備報告

将小瓶平衡缓冲液的全部内容物添加到大瓶苯酚：氯仿：异戊醇中。 在使用前轻柔地混合并使相实现分离，大约 2-4 小时。 这会将苯酚溶液的 pH 值从 6.7± 0.2 调整到 8.0± 0.2。

其他說明

添加平衡缓冲液后，产品可稳定 6 个月，而不添加缓冲液可稳定 2 年。 在添加平衡缓冲液后，在 -20 °C 下避光储存可将保质期延长至 1 年以上。

相關產品

产品编号
说明
价格

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

標靶器官

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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实验方案

Reverse Transcription Protocol

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

逆转录实验方案

将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。

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