N4662

Nek6, Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• 别名: Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
• MDL编号: MFCD07370972
• UNSPSC代码: 12352200
• NACRES: NA.32

属性

• 重组: expressed in E. coli
• 质量水平: 100
• 方案: ≥80% (SDS-PAGE)
• 表单: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• 分子量: protein apparent mol wt ~58 kDa
• UniProt登记号: Q9HC98
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... NEK6(10783)

说明

应用

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

生化/生理作用

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

单位定义

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

外形

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable