There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.
推荐产品
产品名称
尼罗红, Technical grade
等级
technical grade
表单
powder
组成
Carbon: 70.0-77.3%
Nitrogen: 7.5-8.8%
技术
titration: suitable
颜色
faint green to brown
mp
203-205 °C (lit.)
溶解性
methanol: 1 mg/mL
λmax
553 nm
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
InChI
1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChI key
VOFUROIFQGPCGE-UHFFFAOYSA-N
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一般描述
尼罗河红是一种亲脂性荧光染料，通常用于各种科学应用，特别是在脂质研究中。它具有染色和可视化细胞和组织中的脂滴的能力。
应用
尼罗河红广泛用于脂质代谢、脂滴动力学、细胞或组织中的脂质积累以及基于脂质的药物传递系统等相关研究。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
其他客户在看
D L Sackett et al.
Analytical biochemistry, 167(2), 228-234 (1987-12-01)
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for
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