生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 80-85 kDa
种属反应性
rat, human, mouse
包装
antibody small pack of 25 μL
浓度
~1 mg/mL
技术
immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton
immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates
western blot: 0.5-1 μg/mL using Jurkat cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MLH1(4292)
mouse ... Mlh1(17350)
rat ... Mlh1(81685)
一般描述
MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.[1]
MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).
免疫原
synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.
应用
- Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化/生理作用
MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.
MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).
目标描述
MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Hereditary nonpolyposis colorectal cancer (HNPCC)/Lynch syndrome
Steinke V. et al.
Deutsches Arzteblatt International, 110(3), 32-32 (2013)
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
Wang Y, et al.
Genes & Development, 14(8), 927-939 (2000)
Association between MutL homolog 1 polymorphisms and the risk of colorectal cancer: a meta-analysis.
Haiyan Chen et al.
Journal of cancer research and clinical oncology, 141(12), 2147-2158 (2015-05-20)
As one of the most essential components of mismatch repair system, MutL homolog 1 (MLH1) plays an increasingly implicated role in initiation and promotion of colorectal carcinogenesis, with germ-line mutations in different loci. However, whether a single genetic variant in
Shinichiro Fukuhara et al.
Oncotarget, 5(22), 11297-11307 (2014-12-20)
Mismatch repair (MMR) enzymes have been shown to be deficient in prostate cancer (PCa). MMR can influence the regulation of tumor development in various cancers but their role on PCa has not been investigated. The aim of the present study
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