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出版信息
J.F. Sambrook and D.W. Russell, ed., Cold Spring Harbor Laboratory Press, 2001, 2100 pp., soft cover
制造商/商品名称
Cold Spring Harbor
一般描述
In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. It includes 240 laboratory protocols in DNA science in which over 35% were created especially for this edition, along with coverage of bioinformatics and DNA microarrays.
目录
Chapter 1. Plasmids and Their Usefulness in Molecular Cloning
Chapter 2. Bacteriophage lambda and Its Vectors
Chapter 3. Working with Bacteriophage M13 Vectors
Chapter 4. Working with High-capacity Vectors
Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA
Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction
Chapter 9. Preparation of Radiolabeled DNA and RNA Probes
Chapter 10. Working with Synthetic Oligonucleotide Probes
Chapter 11. Preparation of cDNA Libraries and Gene Identification
Chapter 12. DNA Sequencing
Chapter 13. Mutagenesis
Chapter 14. Screening Expression Libraries
Chapter 15. Expression of Cloned Genes in Escherichia coli
Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells
Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells
Chapter 18. Protein Interaction Technologies
Chapter 2. Bacteriophage lambda and Its Vectors
Chapter 3. Working with Bacteriophage M13 Vectors
Chapter 4. Working with High-capacity Vectors
Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA
Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction
Chapter 9. Preparation of Radiolabeled DNA and RNA Probes
Chapter 10. Working with Synthetic Oligonucleotide Probes
Chapter 11. Preparation of cDNA Libraries and Gene Identification
Chapter 12. DNA Sequencing
Chapter 13. Mutagenesis
Chapter 14. Screening Expression Libraries
Chapter 15. Expression of Cloned Genes in Escherichia coli
Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells
Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells
Chapter 18. Protein Interaction Technologies
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