推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
分子量
antigen 45 kDa
种属反应性
human, rat, mouse
技术
flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells.
immunocytochemistry: 1:1,000 using HeLa cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma
immunoprecipitation (IP): 1:50
western blot: 1:1,000 using serum-treated NIH-3T3 cells
UniProt登记号
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer217/pSer221)
基因信息
human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)
一般描述
MAP激酶激酶（MAPKK、MEK1和MEK2）是对苏氨酸/酪氨酸残基双磷酸化激活底物的酶家族成员。MEK1和MEK2的催化结构域有86%的同源性。MEK1/2先被丝裂原刺激细胞中的Raf-1激酶活化， MEK1/2随后激活ERK1/2，进而激活多个下游靶标，参与细胞增殖、凋亡和移动。除了ERK1/2，可能还有其他非催化性的MEK1/2效应子。MEK的各种亚型在中枢神经系统、胸腺、脾脏、心脏、肺和肾脏中广泛表达。 活性MEK1/2是NIH3T3细胞转化和PC-12细胞分化的充分条件。
该抗体仅特异性识别Ser217和Ser221位磷酸化的MEK1/2 (45 kDa)。它与仅Ser217为磷酸化的MEK1/2反应强烈，但与仅Ser221磷酸化的MEK1/2反应较弱。与其他相关家族成员（包括SEK）没有交叉反应。
该抗体仅特异性识别Ser217和Ser221位磷酸化的MEK1/2 (45 kDa)。它与仅Ser217为磷酸化的MEK1/2反应强烈，但与仅Ser221磷酸化的MEK1/2反应较弱。与其他相关家族成员（包括SEK）没有交叉反应。
特异性
特异性识别Ser217和Ser221位磷酸化的MEK1/2。不与非磷酸化MEK 1/2、SEK (MKK4)、MKK3或MKK6反应。
免疫原
对应人MEK 1/2中围绕Ser217/221的一段残基的合成磷酸肽Ser217/221
应用
抗-磷酸MAP激酶激酶1/2(MEK1/2)抗体应用广泛。对于血清处理的NIH3T3细胞的免疫印迹分析，推荐的工作稀释度为1:1000。对于免疫沉淀，可使用1:50的工作稀释度。对于石蜡包埋的人乳腺癌切片的免疫组织化学检测，可使用1:100的抗体稀释液。对于HeLa细胞的免疫细胞化学应用（免疫荧光），可使用1:1000的工作稀释度。
外形
10 mM HEPES、pH 7.5溶液，含有150 mM氯化钠、100 μg/ml牛血清白蛋白和50%甘油。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Hyukjin Cha et al.
The Biochemical journal, 378(Pt 3), 857-865 (2003-12-13)
Phosphorylation-specific antibodies provide a powerful tool for analysing the regulation and activity of proteins in the MAP (mitogen-activated protein) kinase and other signalling pathways. Using synchronized cells, it was observed that phosphorylation-specific antibodies developed against the active form of MKK1/MKK2
S Cowley et al.
Cell, 77(6), 841-852 (1994-06-17)
The MAP kinase pathway is activated by a wide variety of external signals leading to cell proliferation or differentiation. However, it is not clear whether activation of this pathway is required for cellular responses or whether it is only one
S J Mansour et al.
Science (New York, N.Y.), 265(5174), 966-970 (1994-08-12)
Mitogen-activated protein (MAP) kinase kinase (MAPKK) activates MAP kinase in a signal transduction pathway that mediates cellular responses to growth and differentiation factors. Oncogenes such as ras, src, raf, and mos have been proposed to transform cells by prolonging the
Jan Pinkas et al.
Cancer research, 62(16), 4781-4790 (2002-08-17)
Activation of the mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK)-mitogen-activated protein kinase (MAPK) pathway is a frequent event in tumorigenesis, and analysis of human breast carcinomas demonstrates that 25-50% of these tumors express elevated levels of activated MAPK1/2. However, a direct
M Raman et al.
Oncogene, 26(22), 3100-3112 (2007-05-15)
Mitogen-activated protein kinases (MAPKs) regulate diverse cellular programs including embryogenesis, proliferation, differentiation and apoptosis based on cues derived from the cell surface and the metabolic state and environment of the cell. In mammals, there are more than a dozen MAPK
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