推荐产品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
Men-8, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~75 kDa
物種活性
mouse, human, rat, monkey
包裝
antibody small pack of 25 μL
技術
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)
一般說明
Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain.
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Men-8 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminus of human MeCP2.
免疫原
synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2.
應用
Monoclonal Anti-MeCP2 antibody has been used:
- in western blotting
- in immunoblotting
- in flow cytometry
- in immunohistofluorescence
- in immunocytochemistry
生化／生理作用
MeCP2 (methyl-CpG binding protein 2) silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes.
Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT).
外觀
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
A codon-optimized Mecp2 transgene corrects breathing deficits and improves survival in a mouse model of Rett syndrome
Matagne V, et al.
Neurobiology of Disease, 99, 1-11 (2017)
Ube3a mRNA and protein expression are not decreased in Mecp2R168X mutant mice
Lawson-Yuen A, et al.
Brain Research, 1180, 1-6 (2007)
Rett syndrome mutations abolish the interaction of MeCP2 with the NCoR/SMRT co-repressor
Lyst M J, et al.
Nature Neuroscience, 16(7), 898-898 (2013)
Tugba G Kucukkal et al.
Biochemistry, 54(41), 6357-6368 (2015-09-30)
Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently
Gang Meng et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(4), 3009-3015 (2015-01-24)
Methyl-CpG-binding protein 2 (MeCP2) is essential in human brain development and has been linked to several cancer types and neuro-developmental disorders. This study aims to screen the MeCP2 related differentially expressed genes and discover the therapeutic targets for osteosarcoma. CCK8
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门