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生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~40 kDa
物種活性
human, mouse (predicted), rat (predicted)
包裝
antibody small pack of 25 μL
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
~2 mg/mL
技術
western blot: 0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... CAB39(51719)
mouse ... Cab39(12283)
rat ... Cab39(301574)
一般說明
MO25 is a 40 kDa scaffolding protein that interacts with STRADα and LKB1 to regulate tumor suppression. MO25 also facilitates the activation and cytoplasmic localization of LKB1 . Anti-MO25 (C-terminus) antibody detects human MO25 (approx. 40 kDa). The product is also predicted to react with rat and mouse MO25. Staining of the MO25 band in immunoblotting is specifically inhibited by the immunizing peptide.
免疫原
synthetic peptide corresponding to amino acids 323-341 located near the C-terminus of human MO25. This sequence is identical in chicken, dog, and bovine MO25 and highly conserved in rat and mouse MO25 (2 amino acid substitutions).
應用
Anti-MO25 (C-terminus) antibody is suitable for use in western blot (0.5-1 μg/mL using HEK-293T cells expressing human MO25 and in PC12 cell lysates).
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Science (New York, N.Y.), 326(5960), 1707-1711 (2009-11-07)
The LKB1 tumor suppressor is a protein kinase that controls the activity of adenosine monophosphate-activated protein kinase (AMPK). LKB1 activity is regulated by the pseudokinase STRADalpha and the scaffolding protein MO25alpha through an unknown, phosphorylation-independent, mechanism. We describe the structure
The EMBO journal, 22(19), 5102-5114 (2003-10-01)
Mutations in the LKB1 protein kinase result in the inherited Peutz Jeghers cancer syndrome. LKB1 has been implicated in regulating cell proliferation and polarity although little is known about how this enzyme is regulated. We recently showed that LKB1 is
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