Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
(NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
• Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
• While gently stirring the water, add 100 ml of 10X medium.
• To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
• While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
• Bring medium to final volume with additional tissue culture grade water.
• It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
• Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
推荐产品
product name
Eagle最低必需培养基, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
品質等級
無菌
sterile-filtered
形狀
liquid
濃度
10 ×
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
成分
phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no
運輸包裝
ambient
儲存溫度
2-8°C
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产品编号
说明
价格
補充物
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?
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