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一般說明
凝集素是植物来源的蛋白质,其具有结合某些神经元表面糖蛋白和糖脂的能力。他们的这种能力有助于它们被用作神经解剖学示踪剂,因为它们以逆行或顺行方式被采集和运输。
應用
来自美洲商陆(商陆)的凝集素用于增殖测定。(使用常规测定,通过在新合成的DNA中掺入 3H 胸苷来测量细胞增殖反应)。
生化/生理作用
凝集素是碳水化合物结合蛋白,对糖部分具有高度特异性。商陆凝集素(PL)是对含有N-乙酰氨基葡糖的糖特异的凝集素,其通过与细胞表面结合来刺激外周淋巴细胞进行有丝分裂。从商陆(美洲商陆)的根中分离出四种类型的凝集素。它们含有同源结构域,但具有不同的分子大小和生物学特性。
PWM 从商陆根中提取。该蛋白质具有红血球凝集、白血球凝集和促有丝分裂特性。PWM 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺低聚物的亲和性。
品質
部分纯化的 TCA 沉淀。
基本不含盐。
基本不含盐。
分析報告
通过将 BrdU 掺入 HeLa 细胞培养基中测定有丝分裂促进活性。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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