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Merck

L8777

Sigma-Aldrich

来自美洲商陆(商陆)的凝集素

lyophilized powder, BioReagent, suitable for cell culture

别名:

PWM, 美洲商陆有丝分裂原

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

無菌

aseptically filled

品質等級

產品線

BioReagent

形狀

lyophilized powder

效力

≤0.3 μg/mL mitogenic activity

技術

cell culture | mammalian: suitable

溶解度

PBS: 1 mg/mL

儲存溫度

2-8°C

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一般說明

凝集素是植物来源的蛋白质,其具有结合某些神经元表面糖蛋白和糖脂的能力。他们的这种能力有助于它们被用作神经解剖学示踪剂,因为它们以逆行或顺行方式被采集和运输。

應用

来自美洲商陆(商陆)的凝集素用于增殖测定。(使用常规测定,通过在新合成的DNA中掺入 3H 胸苷来测量细胞增殖反应)。

生化/生理作用

凝集素是碳水化合物结合蛋白,对糖部分具有高度特异性。商陆凝集素(PL)是对含有N-乙酰氨基葡糖的糖特异的凝集素,其通过与细胞表面结合来刺激外周淋巴细胞进行有丝分裂。从商陆(美洲商陆)的根中分离出四种类型的凝集素。它们含有同源结构域,但具有不同的分子大小和生物学特性。
PWM 从商陆根中提取。该蛋白质具有红血球凝集、白血球凝集和促有丝分裂特性。PWM 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺低聚物的亲和性。

品質

部分纯化的 TCA 沉淀。
基本不含盐。

分析報告

通过将 BrdU 掺入 HeLa 细胞培养基中测定有丝分裂促进活性。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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