This product is assigned retest date of approximately 3 years from the date of Quality Release. This date is lot-specific and may be found in the product Certificate of Analysis. When stored at room temperature, in a tightly sealed container, the material will be stable at least until the assigned retest date.
$211.00
请联系客服了解存货情况
新价格，新优惠！
推荐产品
等级
Molecular Biology
for molecular biology
无菌性
non-sterile
表单
tablet
技术
microbiological culture: suitable
应用
food and beverages
microbiology
储存温度
room temp
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。 这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。
脱水Luria-Bertani (LB)培养基称为Lennox LB，以氨基酸作为碳源。[1]
应用
适用于 大肠杆菌 菌株的非选择性培养，用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养，包括需要低盐条件的抗生素，如吉欧霉素®。
特点和优势
Lennox LB平板电脑提供：
- 方便的小包装，无需称重
- 快速准备–无需搅拌
组分
10 g/L胰蛋白胨
5克/升酵母提取物
5 g/L 氯化钠
2.2克/升惰性粘合剂
5克/升酵母提取物
5 g/L 氯化钠
2.2克/升惰性粘合剂
重悬
将每片1.1g片剂加入48.3毫升水中（无需搅拌）。在121℃高压灭菌20分钟。添加抗生素（如果需要）之前，请冷却。
法律信息
Zeocin is a registered trademark of Cayla Sarl
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Miller, J.H.
Experiments in Molecular Genetics null
Escherichia coli Physiology in Luria-Bertani Broth
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746-8746 (2007)
Synthesis of antibacterial surfaces by plasma grafting of zinc oxide based nanocomposites onto polypropylene
De Rancourt Y, et al.
Journal of Colloid and Interface Science, 402, 320-326 (2013)
Simultaneous quantification of active carbon-and nitrogen-fixing communities and estimation of fixation rates using fluorescence in situ hybridization and flow cytometry
McInnes AS, et al.
Applied and Environmental Microbiology, 80(21), 6750-6759 (2014)
Fluorescence in situ hybridization of ductal lavage samples identifies malignant phenotypes from cytologically normal cells in women with breast cancer
Adduci KM, et al.
Cancer Cytopathology, 111(3), 185-191 (2007)
实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
-
How long can these tablets be stored at room temperature in the bottle (after opening).
1 answer-
Helpful?
-
-
Do the tablets contain any additives to adjust the pH of the media? Also, what should the final pH of the media be?
1 answer-
The pH of the media can be 7.0, but depending on the application, it can range from 7.0 to 8.0. The pH can be adjusted using 1N NaOH and 1N HCl.
Helpful?
-
-
Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
Helpful?
-
-
Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
-
-
What is the difference among the many LB - Lennox products?
1 answer-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
