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L6007

Sigma-Aldrich

亮氨酸氨基肽酶，微粒体 来源于猪肾脏

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

别名:

亮氨肽酶, 亮酰胺氨肽酶, 氨基肽酶 M, 氨肽酶M, 氨肽酶N, 组织蛋白酶 III

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25 UNITS
$773.00
50 UNITS
$1,270.00
100 UNITS
$2,310.00

$773.00

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25 UNITS
$773.00
50 UNITS
$1,270.00
100 UNITS
$2,310.00

About This Item

CAS号:
9054-63-1
EC 号:
3.4.11.2 (BRENDA, IUBMB)
EC 号:
232-942-5
MDL编号:
MFCD00131501
UNSPSC代码:
12352204
NACRES:
NA.54

$773.00

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生物来源

Porcine kidney

类型

Type VI-S

表单

lyophilized powder

比活

≥12 units/mg protein (biuret)

分子量

320 kDa

组成

Protein, 30-70%

储存温度

−20°C

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相关类别

一般描述

来自猪的亮氨酸氨肽酶（LAP）是存在于胞质溶胶中的一种320-kDa外肽酶。[1]它的结构中含有锌，属于M1和M17肽酶家族。[2]LAP具有双叶结构并在C末端具有催化结构域以及一个可变的N末端结构域。[3]

应用

亮氨酸氨肽酶，来自猪肾的微粒体已用于腺癌细胞系ES-2中氨肽酶N（APN）抑制剂的筛选检测[4]以及APN抑制剂的抑制动力学研究中。[5][6]

生化/生理作用

亮氨酸氨肽酶（LAP）可在N-末端的亮氨酸残基处催化蛋白的水解。[1]LAP水平的改变与肿瘤的增殖和进展有关。LAP是卵巢上皮癌治疗的一种潜在生物标志物。[2] 猪LAP可水解甘氨酸和精氨酸底物。[3]

单位定义

一个单位在37°C 下 pH 7.2 时每分钟将水解 1.0μ摩尔 L-亮氨酸-对硝基苯胺为 L -亮氨酸和对硝基苯胺（25°C 下可获得活性约为37°C 下的 40%）。以 L-亮氨酸对硝基苯胺为底物得到的活性是以 L-亮氨酸酰胺为底物得到的活性的 2-5 倍。

外形

主要含有磷酸盐缓冲盐，pH 7.0

抑制剂

产品编号
说明
价格

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000313108
0000313107
0000313108
0000313107
0000280242

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Refolding of hexameric porcine leucine aminopeptidase using a cationic detergent and dextrin-10 as artificial chaperones
Laslo AC, et al.
Journal of Biotechnology, 140(3-4), 162-168 (2009)
Activity screening and structure-activity relationship of the hit compounds targeting APN/CD13
Wang X, et al.
Fundamental & Clinical Pharmacology, 25(2), 217-228 (2011)
Novel and highly sensitive fluorescent assay for leucine aminopeptidases
Huang H, et al.
Analytical biochemistry, 391(1), 11-16 (2009)
Leucine aminopeptidases: diversity in structure and function
Matsui M, et al.
Biological Chemistry, 387(12), 1535-1544 (2006)
The effect of different species Aminopeptidase N Structure on the activity screening of Aminopeptidase N Inhibitor
Wang X, et al.
Biological & Pharmaceutical Bulletin, 33(10), 1658-1665 (2010)
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Questions

1–2 of 2 Questions  

  1. I would like to know the optimum solvent for dissolving, storing and using this reagent (Cat # L6007-25UN).

    1 answer

    1. According to the Enzymatic Assay for Leucine Aminopeptidase, Microsomal Enzyme Solution, prepare a solution containing 0.10 - 0.15 unit/ml of Leucine Aminopeptidase, Microsomal in cold deionized water immediately before use.

      More information can be found at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/356/218/l6007enz.pdf.

      This formulation contains phosphate buffer salts. For reconstitution, storage, and use of this product, 50 mM Sodium Phosphate Buffer, pH7.2, is a suitable solvent.

      Helpful?

  2. I recently purchased Leucine Aminopeptidase from porcine kidney Cat # L6007-25UN. I'd like to inquire how many mgs of protein is in one vial.

    1 answer

    1. The milligrams of protein per vial will depend on the units/mg protein defined on the product's certificate of analysis. For example, lot SLCM3898 reports specific activity of 19 units/mg protein. This results in approximately 1.3 mg of protein per vial for this particular lot. The protein content of this lot of material is reported at 61%, therefore the total mass of solid material in the 25UN vial will total around 2.2 mg.

      (Link to certificate of analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L6007-BULKSLCM3898.pdf)

      Helpful?

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