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L5900

Sigma-Aldrich

亚油酸-水溶性

powder, BioReagent, suitable for cell culture

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10 MG
$107.00

$107.00


国内现货,预计发货时间2025年4月11日详情


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10 MG
$107.00

About This Item

UNSPSC代码:
12352211
NACRES:
NA.75

$107.00


国内现货,预计发货时间2025年4月11日详情


获取大包装报价

生物来源

plant (Safflower oil)

产品线

BioReagent

表单

powder

技术

cell culture | mammalian: suitable

溶解性

H2O: 50 mg/mL

储存温度

−20°C

应用

水溶性亚油酸已被用作脂肪培养基的成分,用于体外诱导原代人肝细胞 (PHH) 的脂肪变性。[1]

生化/生理作用

亚油酸(LA)是花生四烯酸的前体,[2]存在于植物油中。[3]它可改善糖尿病大鼠的伤口愈合。[2]LA触发肿瘤和大肠癌细胞的凋亡。[3]脂肪组织和血小板中的LA水平与冠状动脉疾病有关。[4]亚油酸与牛血清白蛋白(BSA)[5]或环糊精等分子结合,可用于细胞培养。[6]

其他说明

每克约含 30 mg 亚油酸;甲基-β 平衡-环糊精。
基于亚油酸含量的包装规格。

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警示用语:

Warning

危险声明

预防措施声明

危险分类

Eye Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

不饱和脂肪酸、油酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

相关内容

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Questions

1–4 of 4 Questions  
  1. If we dissolve the powder in distilled water, as described in one of the questions, can we aliquot this solution and freeze it at -20 or -70. If so, for how long? Or should we make a fresh solution every time?

    1 answer
    1. This product has not been tested for stability once dissolved in water. It is recommended to reconstitute just before use.

      Helpful?

  2. Hi there, could you confirm the validity of our stock preparation for L5900-10MG? We dissolve the full 10 mg tube in 1.43 mL distilled water, yielding a 25 millimolar stock solution.

    1 answer
    1. Linoleic acid has a molecular weight of 280.45 g/mol. Dissolving the full 10 mg tube in 1.43 mL of distilled water yields a 25 millimolar stock solution.

      Helpful?

  3. Dear, can you please clarify the composition of this product for me? In my understanding this product contains minimum 2.5% linoleic acid encapsulated in methyl-β-cyclodextrin.

    1 answer
    1. The product contains approximately 30 mg of linoleic acid per gram; balance methyl-?-cyclodextrin.
      Package size is based on linoleic acid content.

      More information can be found in the Data Sheet below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdf

      Helpful?

  4. How to prepare FAME from it?

    1 answer
    1. This product is designed for use in mammalian cell culture applications as a supplement. If you are seeking a protocol for derivatization of Fatty Acids to FAMEs, please see the following: https://www.sigmaaldrich.com/technical-documents/protocol/food-and-beverage-testing-and-manufacturing/chemical-analysis-for-food-and-beverage/derivatization-of-fatty-acids-to-fames

      Helpful?

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