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Merck
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L4655

Sigma-Aldrich

乳胶珠,羧酸盐改性聚苯乙烯,荧光黄绿色

aqueous suspension, 1.0 μm mean particle size

别名:

Fluorescent Latex Beads

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1 ML
$98.00

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1 ML
$98.00

About This Item

MDL號碼:
分類程式碼代碼:
12162002
NACRES:
NA.25

$98.00


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形狀

aqueous suspension

品質等級

成份

Solids, 2.5%

技術

cell based assay: suitable

平均粒徑

1.0 μm

螢光

λex ~470 nm; λem ~505 nm

適合性

suitable for immunoassay

應用

cell analysis

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應用

乳胶珠、羧酸盐改性聚苯乙烯、荧光黄绿色可以用于:
  • 表皮生长因子缀合物制备,用于间接免疫荧光成像研究[1]
  • 吞噬作用成像研究之前培养小鼠支气管肺泡巨噬细胞和人单核细胞衍生的巨噬细胞[2]
  • 与沙门氏菌表达的绿色荧光蛋白(GFP)并用,用于共聚焦显微镜研究和生物荧光成像[3]

羧基化改性聚苯乙烯乳胶珠被用于收集有关 Carabus lefebvrei(以蜗牛为食的意大利特有步甲,通常用作亚平宁山脉森林栖息地质量的指标)免疫系统的形态功能数据。羧基化改性聚苯乙烯乳胶珠也被用于研究比较鞘翅目Cetonischema aeruginosa成虫和幼虫的免疫防御机制、研究培养的大鼠甲状腺细胞的吞噬活性。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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J Ablin et al.
Apoptosis : an international journal on programmed cell death, 10(5), 1009-1018 (2005-09-10)
A number of mechanisms have been proposed to explain the etiology of drug-induced lupus (DIL) but the effect of apoptotic and necrotic cell handling has not been previously examined. To evaluate the effect of quinidine and procainamide at therapeutic range
Po-Tsang Lee et al.
Animals : an open access journal from MDPI, 11(8) (2021-08-28)
We investigated whether phycoerythrin (PE), a pigment sourced from marine algae, could act as an immunomodulatory agent in whiteleg shrimp (Litopenaeus vannamei). To this end, PE was extracted and purified from a PE-rich macroalgae, Colaconema sp. Our in vitro analysis
Bin Fan et al.
Journal of neuroimmunology, 244(1-2), 45-50 (2012-01-21)
Meningothelial cells (MECs) form the innermost layer of the meningeal sheath and as such are in direct contact with the cerebrospinal fluid (CSF) likely influencing CSF composition. The CSF space is a site of active immunological processes. To investigate an
Cell surface epidermal growth factor receptors increase Src and c-Cbl activity and receptor ubiquitylation
Parks PE and Ceresa BP
The Journal of Biological Chemistry, 289(37), 25537-25545 (2014)
Elin Silverpil et al.
Inflammation, 34(1), 1-9 (2010-03-27)
There is now substantial evidence that the cytokine interleukin-17 orchestrates the accumulation of neutrophils in mammals and thereby contributes to host defense. However, the role of IL-17 in controlling neutrophil turnover is not fully understood. Here, we demonstrate that IL-17

Questions

1–5 of 5 Questions  
  1. What is the concentration of product L4655?

    1 answer
    1. The concentration of the Latex Beads is approximately 4.75x10^10 beads/mL.

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  2. What are the ingredients of the solution in which the plastic is diluted? Can I dilute it in PBS?

    1 answer
    1. This product is prepared in water containing a prioprietary anionic surfactant and >0.1% Sodium Azide. Phosphate Buffered Saline is a suitable diluent.

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  3. Are there latex beads recommended for phagocytosis, and if so, what size is recommended?

    1 answer
    1. There is no performance testing of latex beads for phagocytosis. Information found indicates that internally, there are no specific protocols or recommendations for a phagocytosis method using the listed latex beads. However, references to literature where the products have been used in research are provided. The maximum excitation and emission for one of the latex bead products, L3280, are also mentioned. Additionally, three products (catalog numbers 59769, 43302, and 89904) were found on the company website, with variations in bead size and fluorescence. For more details, you may refer to the provided links and Knowledge Article 000007596.

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  4. What are the differences between carboxylate-modified polystyrene and amine-modified polystyrene Latex beads, and which type of beads is suitable for phagocytosis applications?

    1 answer
    1. When selecting beads for phagocytosis assays, there are three key parameters to consider: the size of the bead, the color of the bead (dyed or undyed), and the modification of the bead (carboxylate, amine, or sulfate modified).

      Beads chosen for phagocytosis experiments typically range from approximately 0.5 to 3 micrometers in size. A 1:250 dilution of the beads into the culture medium is commonly used for phagocytosis experiments. Latex Beads, 2 um (Product No. L0905), can be utilized for phagocytosis assays, as macrophages can phagocytose particles larger than 2 um.

      Fluorescent latex beads are available with different fluorescent dyes incorporated into the beads. The yellow-green beads are commonly used for phagocytosis studies and are compatible with most flow cytometers and fluorescent microscopes.

      For phagocytosis applications, the choice of bead modification (carboxylate vs. amine) depends on the specific assay being conducted. Carboxylate-modified beads, with a negative charge, mimic the uptake of apoptotic cells, while amine-modified beads, with a positive charge, are used to mimic the uptake of opsonized cells.

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  5. What is the recommended storage temperature?

    1 answer
    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

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