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Merck

L2637

Sigma-Aldrich

脂多糖 来源于大肠杆菌 055:B5

purified by gel-filtration chromatography

别名:

LPS

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Escherichia coli (O55:B5)

品質等級

形狀

lyophilized powder

純化經由

gel-filtration chromatography

雜質

<3% Protein

顏色

white to faint gray

溶解度

water: soluble

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

这种产品是从大肠杆菌中提取的大肠杆菌血清型O55:B5,通过凝胶过滤纯化。源菌株是CDC 1644-70。脂多糖O55:B5已被用于以1纳克/毫升刺激人腹膜巨噬细胞,并以1-100纳克/毫升刺激马腹膜巨噬细胞。

應用

脂多糖(LPS)是革兰氏阴性细菌细胞壁的特征组分。脂多糖及其脂质 A 部分通过 Toll 样受体 4(TLR4)刺激先天免疫系统的细胞,Toll 样受体 4是 Toll 样受体蛋白家族的成员,其识别常见的病原体相关分子模式(PAMP)。

生化/生理作用

脂多糖位于膜的外层,在非包膜菌株中,暴露在细胞表面。它们有助于外膜的完整性,保护细胞免受胆盐和亲脂性抗生素的作用。

準備報告

该产品可溶于水(5毫克/毫升)或细胞培养基(1毫克/毫升),产生一种朦胧的淡黄色溶液。在涡旋并升温至70-80 oC温度后,在盐水中获得了更浓但仍模糊的溶液(20毫克/毫升)脂多糖是在每种溶剂中形成胶束的分子。在水和磷酸盐缓冲盐水中观察到混浊的溶液。有机溶剂不能提供更清晰的溶液。甲醇产生带有漂浮物的混浊悬浮液,而水产生均匀混浊的溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Skull and crossbones

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 2 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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