I9781

Importin β1 human

≥80% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), buffered aqueous glycerol solution

• 别名: Importin β, Karyopherin β1, p97
• MDL编号: MFCD03457282
• UNSPSC代码: 12352202
• NACRES: NA.32

属性

• 重组: expressed in E. coli (N-terminal histidine tagged)
• 方案: ≥80% (SDS-PAGE)
• 表单: buffered aqueous glycerol solution
• 分子量: ~97 kDa by SDS-PAGE
• UniProt登记号: Q14974
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... KPNB1(3837)

说明

一般描述

Importin β1 has 19 tandemly repeated HEAT (huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A) and the yeast kinase TOR1) motifs.[1] The gene is mapped to human chromosome 17q21.[2] The protein shuttles between the cytoplasm and nucleus. It belongs to the importin β family of proteins.[3]

生化/生理作用

Import receptor, prototype of the nuclear transport receptor family, which interact directly with nuclear pore complex (NPC) and mediate nucleocytoplasmic transport.

Importin β, an import receptor, is a prototype of the nuclear transport receptor family, which comprises in human more than 20 proteins (90-180 kDa). These proteins interact directly with nuclear pore complex (NPC) and mediate nucleocytoplasmic transport. Importin β imports into the nucleus proteins carrying canonical nuclear localization signals (NLS) as well as UsnRNAs, which it binds via adaptor proteins, importin α and snurpotin-1 respectively. Importin β also binds directly, without adaptors, ribosomal proteins, Smad proteins, and virus derived proteins such as HIV Rev and Tat, that contain non classical NLS. In addition, Improtin b has been shown to be involved in the nuclear envelope assembly process.
The mechanism of importin β action in nuclear import can be demonstrated by the well-studied import of proteins containing classical NLS. Importin β forms a complex with Importin α, which, in turn, binds the cargo protein via its NLS. The Impβ/Impα/cargo complex translocates into the nucleus. When the complex reaches the nuclear site of the NPC, Ran-GTP binds the Impβ to form Impβ/Ran-GTP complex and released the Impα and the cargo protein. The Impβ/Ran-GTP complex is then exported to the cytoplasm where the complex dissociates upon hydrolysis of GTP to GDP, making Impβ ready for a new import cycle.

外形

Solution in 5% glycerol containing 20 mM Hepes, pH 7.5, 110 mM potassium-acetate, 2 mM magnesium-acetate, 0.5 mM EGTA, 0.1 mM ATP, 2 mM DTT, and protease inhibitors.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: nwg
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)