The activity of the product should be 25 IU/mL, based on the formulation and the IU of the raw materials used. The product has a specification of >= 0.9mg/mL, and the raw material has an activity specification of >= 25 IUM. Therefore, depending on the lot the customer received, if the assay of the lot they received was 1mg/mL, then the activity would be 25 IU/mL.
推荐产品
无菌性
sterile-filtered
产品线
BioReagent
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
运输
ambient
储存温度
2-8°C
相关类别
一般描述
无血清培养可避免血清带来的成分差异和污染。无血清配方将通常由血清提供的因子替换为纯化形式，适合许多体外生长和分化研究。这些纯化因子包括胰岛素、转铁蛋白、硒、丙酮酸和乙醇胺。其他补充成分并不一定，具体取决于研究的细胞类型和所用的基础培养基。ITS补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠的混合物。它是一种通用的细胞补充剂，设计用于含有丙酮酸钠的非复合型培养基（如MEM、RPMI-1640）及复合型培养基（如Ham′s F-12、DME/F-12、MEM）。
应用
ITS+1液体培养基补充剂(100×)已用作高糖杜氏改良Eagle培养基(DMEM)的补充剂，用于小鼠间充质干细胞(mMSC)向软骨细胞的分化。还用作基础分化培养基的补充剂，用于在无血清条件下使人多能诱导干细胞(hiPSC)分化为成骨细胞。
组分
含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、50 mg/mL 牛血清白蛋白和 470μg/ml 亚油酸（100x 浓度）。
制备说明
使用不含酚红的Earle′平衡盐溶液（EBSS）制备。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
其他客户在看
Chang Guo et al.
Molecular nutrition & food research, e1801393-e1801393 (2019-06-07)
Alcoholic liver disease is a serious threat to human health. The development of drug candidates from complementary and alternative medicines is an attractive approach. Myricetin can be found in fruit, vegetables, and herbs. This study investigates the protective effect of
Heng Zhu et al.
Nature protocols, 5(3), 550-560 (2010-03-06)
Unlike humans, mouse bone marrow-derived mesenchymal stem cells (MSCs) cannot be easily harvested by adherence to plastic owing to the contamination of cultures by hematopoietic cells. The design of the protocol described here is based on the phenomenon that compact
Deniz M Özata et al.
Endocrine-related cancer, 18(6), 643-655 (2011-08-24)
Adrenocortical carcinoma (ACC) is an aggressive tumor showing frequent metastatic spread and poor survival. Although recent genome-wide studies of ACC have contributed to our understanding of the disease, major challenges remain for both diagnostic and prognostic assessments. The aim of
Jing Wen et al.
Scientific reports, 10(1), 2782-2782 (2020-02-19)
The developmental competence of IVM porcine oocytes is still low compared with that in their in vivo counterparts. Although many studies reported effects of glucose metabolism (GM) on oocyte nuclear maturation, few reported on cytoplasmic maturation. Previous studies could not
Elena G Popa et al.
Journal of tissue engineering and regenerative medicine, 9(5), 550-563 (2013-01-11)
Due to the limited self-repair capacity of cartilage, regenerative medicine therapies for the treatment of cartilage defects must use a significant amount of cells, preferably applied using a hydrogel system that can promise their delivery and functionality at the specific
商品
Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
棕榈酸等脂肪酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统（用于生物制造包括单克隆抗体在内的异源蛋白）的性能。
How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
相关内容
Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
-
Could you provide information on the unit of activation for the ITS+1 liquid media supplement - product I2521? The media I want to use requires 0.023U/mL of insulin in 500mL of RPMI, and I need to understand the activation units for the insulin supplement. The product information only mentions a 1mg/mL concentration of insulin.
1 answer-
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
