We do not recommend re-using the columns. Each column is intended for one time use.
$250.00
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保质期
2 yr at 2‑8 °C
包装
pkg of 10 ea
pkg of 50 ea
孔径
100 nm pore size
容量
>0.5 mg/column
储存温度
2-8°C
一般描述
试剂盒含有针对每种离心柱的3种组合管。
应用
预填充的即用型HIS-Select离心柱可实现对含有组氨酸标签蛋白的小规格细胞粗提取物进行快速而一致的纯化。该离心柱包含二氧化硅颗粒基质并使用HIS-Select专利的不带电荷的亲水性镍螯合键合化学。HIS-Select离心柱对组氨酸标记的蛋白具有高度选择性，而对其他蛋白的结合特异性极低。这些便捷的离心柱可实现快速的一步法纯化，在不到15分钟的时间内分离出至少150 μg的组氨酸标签蛋白。
特点和优势
- 高选择性确保了高纯度
- 便捷且即用
- 每次使用的一致性蛋白结合
- 兼容CelLytic™细胞裂解试剂
法律信息
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Tian Luo et al.
Clinical and vaccine immunology : CVI, 17(1), 87-97 (2009-12-04)
Recently, major species-specific antibody epitopes in three immunoreactive tandem repeat proteins (TRPs) of Ehrlichia chaffeensis, TRP32, TRP47, and TRP120, have been identified and molecularly characterized within tandem repeat (TR) regions. In this study, we mapped the major immunodeterminants of the
Michael D BeGora et al.
The Journal of biological chemistry, 285(38), 29147-29155 (2010-07-24)
Three sequential methylations of phosphoethanolamine (PEA) are required for the synthesis of phosphocholine (PCho) in plants. A cDNA encoding an N-methyltransferase that catalyzes the last two methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2
Ozan Gundogdu et al.
Journal of bacteriology, 193(16), 4238-4249 (2011-06-07)
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative
Dirk Maass et al.
PloS one, 4(7), e6373-e6373 (2009-07-29)
As the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches that have successfully increased the carotenoid content in agronomically relevant non-green plant tissues through tissue-specific PSY overexpression.
Anne Durand et al.
The Journal of biological chemistry, 281(40), 29558-29567 (2006-07-26)
In Escherichia coli, the ammonia channel AmtB and the P(II) signal transduction protein GlnK constitute an ammonium sensory system that effectively couples the intracellular nitrogen regulation system to external changes in ammonium availability. Binding of GlnK to AmtB apparently inactivates
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Can HIS-Select® Spin Column, Product H7787, be re-used?
1 answer-
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What is the binding capacity of the HIS-Select® Spin Column, Product H7787?
1 answer-
The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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