The detection limit of product H1029 is 0.5 μg/ml or less.
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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
HIS-1, monoclonal
表单
liquid
技术
dot blot: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
同位素/亚型
IgG2a
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
聚组氨酸（小鼠IgG2a）单克隆抗体可识别天然或变性的还原型合成聚组氨酸和聚组氨酸标签融合蛋白。 该抗体优先识别N-末端标签融合蛋白，并且与原核表达载体pET、pRSET和pTrc表达的融合蛋白反应。
特异性
该抗体可识别合成聚组氨酸，以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
免疫原
重组聚组氨酸标签融合蛋白。
应用
该抗体适用于免疫印迹、斑点杂交印迹、ELISA和免疫细胞化学分析。小鼠聚组氨酸单克隆抗体可用于蛋白质免疫印迹分析。
生化/生理作用
IgG2a（免疫球蛋白G 2a）基因主动参与刺激小鼠的自身免疫。该基因在病毒感染中高表达。
外形
以含有15mM叠氮化钠的腹水形式提供。
其他说明
用于免疫印迹的建议稀释度为1:3000
法律信息
本产品受专利DE 19507166以及独家授权给 QIAGEN GmbH（Qiagen Strasse 1, D-40724 Hilden, Germany）的国外同等专利的保护。
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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What is the detection limit of Product H1029, Monoclonal Anti-polyHistidine antibody?
1 answer-
Helpful?
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Is Product H1029, Monoclonal Anti-polyHistidine antibody, suitable for use in immunofluorescence studies?
1 answer-
Product H1029 has been used in immunofluorescence and a good picture of the staining has been published. Ker R. Marshall, et al., Journal of General Virology 83, 1601-1612 (2002).
Helpful?
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How many histidines are required for proper recognition by Product H1029, Monoclonal Anti-polyHistidine antibody?
1 answer-
Product H1029, Monoclonal Anti-polyHistidine antibody requires at least 6 histidines in a row for recognition.
Helpful?
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