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Merck

GLN1

Sigma-Aldrich

谷氨酰胺和谷氨酸检测试剂盒

别名:

L-glutamine enzymatic quantification kit

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1 KIT
$1,030.00

$1,030.00


预计发货时间2026年5月20日详情



选择尺寸

变更视图
1 KIT
$1,030.00

About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

$1,030.00


预计发货时间2026年5月20日详情


用途

 kit sufficient for 50 tests

包装

pkg of 1 kit

储存条件

dry at room temperature

技术

UV/Vis spectroscopy: suitable

λmax

340 nm

应用

cell analysis
detection

储存温度

2-8°C

应用

该试剂盒通过将NAD还原为NADH,在339 nm波长处测定分光光度,用酶法测定L-谷氨酰胺和L-谷氨酸,
检测范围为:0.056-0.56 mM L-谷氨酸;0.14-1.4 mM L-谷氨酰胺。
谷氨酰胺和谷氨酸测定试剂盒用于确定以下物质中L-谷氨酰胺的浓度:
  • 最低必需培养基(MEM)培养基[1]
  • 上清液[2]
  • 血浆 [3]

生化/生理作用

谷氨酰胺/谷氨酸测定试剂盒设计通过L-谷氨酰胺酶促脱氨、L-谷氨酸脱氢以及NAD+ 转为NADH后,用分光光度法测量L-谷氨酰胺和/或L-谷氨酸。该试剂盒可用于分光光度法测定液体制剂中的L-谷氨酰胺和/或L-谷氨酸。

警示用语:

Danger

危险分类

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Tianyu Han et al.
Cell research, 28(6), 655-669 (2018-03-09)
Glutamine metabolism plays an important role in cancer development and progression. Glutaminase C (GAC), the first enzyme in glutaminolysis, has emerged as an important target for cancer therapy and many studies have focused on the mechanism of enhanced GAC expression
Wei Shi et al.
Oncology letters, 14(6), 6881-6887 (2017-11-23)
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
Monitoring for overreaching in rugby league players
Coutts AJ, et al.
European Journal of Applied Physiology, 99(3), 313-324 (2007)
Patricia Martins Bock et al.
Molecular and cellular biochemistry, 411(1-2), 351-362 (2015-11-05)
In this work, we aimed to investigate the effects of long-term supplementations with L-glutamine or L-alanyl-L-glutamine in the high-fat diet (HFD)-fed B6.129SF2/J mouse model over insulin sensitivity response and signaling, oxidative stress markers, metabolism and HSP70 expression. Mice were fed
X Chang et al.
Clinical and experimental dermatology, 42(6), 614-621 (2017-06-10)
Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.

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Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物,但许多肿瘤细胞表现出线粒体活性增加。

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