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描述
Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White
特点
wetted part: no
包装
pkg of 1 kit
制造商/商品名称
Cytiva RPN2108
储存温度
2-8°C
相关类别
一般描述
ECL™ Western Blotting System is the world′s most widely used and referenced chemiluminescent immunodetection system.
ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.
ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.
特点和优势
- Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents.
- Optimized system provides rapid, sensitive results using simple protocols.
- Designed for use with nitrocellulose and PVDF membranes.
- High sensitivity: Up to 10 times more sensitive than colorimetric methods.
- Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
- Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
- Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
- Non-radioactive-no specialized facilities needed
- Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
ECL is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
12 - Non Combustible Liquids
Marie-Theres Zeuner et al.
Biochimica et biophysica acta, 1863(12), 3084-3095 (2016-11-05)
A distinct feature of the Toll-like receptor 4 (TLR4) is its ability to trigger both MyD88-dependent and MyD88-independent signalling, culminating in activation of pro-inflammatory NF-κB and/or the antiviral IRF3. Although TLR4 agonists (lipopolysaccharides; LPSs) derived from different bacterial species have
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Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
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