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Merck

GE28-9574-99

TALON® Superflow

Cytiva 28-9574-99, pack of 10 mL

别名:

TALON Affinity Resin

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About This Item

分類程式碼代碼:
23151817
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

包裝

pack of 10 mL

製造商/商標名

Cytiva 28-9574-99

基質

6% cross-linked agarose

粒徑

60-160 μm

cleaning in place

2-14(Co2+-stripped medium)

工作範圍

3-12(Co2+-stripped medium)

容量

≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

儲存溫度

2-8°C

一般說明

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

特點和優勢

  • Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

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实验方案

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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