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Key Documents

GE17-5168-01

Sigma-Aldrich

Mono S® 5/50 GL

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

材料

PEEK column

描述

Ion Exchanger Type (Methyl sulfonate strong cation)

包裝

pkg of 1 mL

參數

580 psi

床尺寸

5 mm × 50 mm

床體積

1 mL

柱內徑

5.7 mm

基質

monodisperse porous polystyrene/divinyl benzene beads
polymeric

基質活性組

methyl sulfonate phase

樣品平均尺寸

10 μm

cleaning

1-14

工作範圍

2-12

分離技術

cation exchange

一般說明

Mono S® is a strong cation exchanger prepacked with MonoBeads in a Tricorn column. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required. The column has 1 mL bed volume for small-scale purifications.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

特點和優勢

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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法律資訊

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

This page shows how to perform a purification of His-tagged membrane proteins.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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实验方案

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

相关内容

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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