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長度 × 內徑
5 cm × 5 mm
基質
MonoBeads
基質活性組
amphoteric
粒徑
10 μm
相容性
mode of use ion exchange
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一般說明
Mono 色谱柱是高效、pH 稳定的色谱柱,设计用于蛋白质、多肽和多核苷酸的高效离子交换分离。这些色谱柱的独特属性是基于单柱支撑体,即任何色谱支撑体中粒径分布最窄的珠状亲水性材料。这种单分散性允许在相对较低的反向压力下获得较高的流速。
應用
Mono columns are used in peptide mapping and monoclonal antibody purification. Mono P columns have been used to purify homogenous forms of fungal peroxygenase and to investigate separation of DNA restriction fragments.
法律資訊
Mono P is a registered trademark of Cytiva
Biotechnology journal, 4(11), 1619-1626 (2009-07-09)
Extracellular peroxygenase from the agaric fungus Agrocybe aegerita is a versatile biocatalyst that oxygenates various substrates by means of hydrogen peroxide. The enzyme is routinely produced in suspensions of soybean meal and has until now been purified by several steps
Analytical biochemistry, 166(1), 158-171 (1987-10-01)
Separation of DNA restriction fragments by FPLC ion-exchange chromatography on Mono Q and Mono P columns was investigated. The columns were found to be particularly suitable for the separation of fragments up to 500-600 bp long. Larger fragments can also
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