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Merck

GE17-5161-01

HiTrap® SP XL

Cytiva 17-5161-01, pack of 5 × 5 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

sulphopropyl

描述

Ion Exchanger Type (value)

包裝

pack of 5 × 5 mL

製造商/商標名

Cytiva 17-5161-01

參數

<20 mL/min flow rate
42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

6% cross-linked agarose with dextran surface extenders

粒徑

45-165 μm

平均直徑

90 μm

cleaning

3-14

工作範圍

4-13

適合性

suitable for bioprocess medium

一般說明

HiTrap® SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

應用

Q Sepharose XL and SP Sepharose XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

特點和優勢

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

This page covers how and when to use Sepharose XL media for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

相关内容

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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