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GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

别名:

affinity chromatography resin, GST-tagged proteins purification

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56
产品GE17-0756-05监管中,请联系销售询价或购买 联系客户支持

ligand

glutathione

包装

pack of 100 mL

制造商/商品名称

Cytiva 17-0756-05

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

4-13

工作范围

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)

相关类别

一般描述

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

特点和优势

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Sajjan Koirala et al.
Cell reports, 32(3), 107922-107922 (2020-07-23)
Spatiotemporal control of Wnt/β-catenin signaling is critical for organism development and homeostasis. The poly-(ADP)-ribose polymerase Tankyrase (TNKS1) promotes Wnt/β-catenin signaling through PARylation-mediated degradation of AXIN1, a component of the β-catenin destruction complex. Although Wnt/β-catenin is a niche-restricted signaling program, tissue-specific

商品

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

实验方案

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

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相关内容

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

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