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Merck

GE17-0409-01

HiTrap® Chelating High Performance

Cytiva 17-0409-01, pack of 1 × 5 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

iminodiacetic acid

包裝

pack of 1 × 5 mL

製造商/商標名

Cytiva 17-0409-01

參數

42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-14

工作範圍

3-13

一般說明

HiTrap® Chelating HP is prepacked with Chelating Sepharose High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

應用

HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC System

特點和優勢

  • For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
  • Conveniently charge with your metal of choice.
  • Iminidoacetic acid coupled to Sepharose High Performance.
  • Purifying Proteins with exposed histidine groups via metal ion complex formation.
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC System.
  • Packed with Chelating Sepharose High Performance
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

实验方案

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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相关内容

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

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