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特點
autoclavable In wet form (pH 7.0) at 120°C for 30 min
包裝
pack of 100 g
製造商／商標名
Cytiva
參數
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
膨潤
1 g swells to 9-11 mL
粒徑
20-50 μm (dry)
20-80 μm (wet)
工作範圍
2-10
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI 密鑰
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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相关类别
一般說明
Sephadex® G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
特點和優勢
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Small bead size for shorter diffusion distances
- Smallest bead size in the Sephadex® G-50 range
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Sephadex is a registered trademark of Cytiva
儲存類別代碼
13 - Non Combustible Solids
Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure
相关内容
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
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