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Merck

G9277

Sigma-Aldrich

异硫氰酸胍

for molecular biology, ≥99%

别名:

硫氰酸亚脲, 硫氰酸胍

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100 G
$278.00
250 G
$514.00
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$996.00

$278.00


国内现货,预计发货时间2025年4月11日详情


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100 G
$278.00
250 G
$514.00
500 G
$996.00

About This Item

线性分子式:
NH2C(=NH)NH2 · HSCN
CAS号:
分子量:
118.16
Beilstein:
3563461
EC 号:
MDL编号:
UNSPSC代码:
12352107
PubChem化学物质编号:
NACRES:
NA.31

$278.00


国内现货,预计发货时间2025年4月11日详情


获取大包装报价

生物来源

synthetic (organic)

质量水平

等级

for molecular biology

方案

≥99%

表单

powder or crystals

储存条件

(Tightly closed. Dry. )

技术

RNA extraction: suitable

颜色

colorless to white,

mp

120-122 °C (lit.)

溶解性

water: 100 mg/mL, clear, colorless

密度

1.29 g/cm3 at 20 °C (68 °F)

痕量阳离子

Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

应用

agriculture

异质活性

DNase, RNase, none detected

SMILES字符串

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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一般描述

硫氰酸胍(Guanidine thiocyanate),又名硫氰酸亚脲,是一种多用途的化合物,广泛用于分子生物学和生物化学研究。它可作为离液剂,破坏细胞和细胞器膜,促进细胞裂解和细胞器分离。作为强效的蛋白变性剂,对未折叠蛋白的效用更胜盐酸胍。利用这种特性可将核酸与变性蛋白质分离,适于细胞裂解液的核酸纯化。

在核酸纯化中,硫氰酸胍可有效灭活DNA酶和RNA酶,从而防止核酸降解,保证可观产量。这种蛋白变性和酶抑制的双重特性令其成为DNA和RNA研究的重要工具。除了核酸分离,硫氰酸胍还能在RNA和DNA提取实验中用于裂解细胞和灭活病毒颗粒。它不仅能破坏各种膜,还能使RNA酶和DNA酶变性,保护提取的核酸免遭酶降解。功能丰富的硫氰酸胍适合各种分子生物学技术,包括PCR、Northern blotting和Southern blotting。在这些实验中作为重要试剂发挥作用,成功实现核酸的分析和扩增。

应用

胍硫氰酸盐已用于:
  • RNA分离之前裂解大肠腺癌细胞系的[1]
  • 作为三重线粒体DNA (mtDNA) qPCR反应体系中的PCR抑制剂[2]
  • 用于从脑脊液(CSF)中提取DNA的qPCR分析[3]

离液剂和强效变性剂;溶解细胞。

生化/生理作用

胍硫氰酸盐是一种变性剂,常用于RNA分离。它可用作全血样品的储存缓冲液。[4]胍硫氰酸盐可灭活核酸酶,是储存和冷冻粪便样本进行DNA研究的理想选择。[5]它可与苯酚−氯仿联用提取RNA。[6]

特点和优势

  • 用途广泛的表面活性剂,适合细胞生物学和生物化学研究
  • 适于灵敏的分子生物学应用,用于RNA提取
  • 不含DNA酶和RNA酶
  • 经检测确认,重金属污染水平低,适用性广

其他说明

如需了解生化试剂系列的更多信息,请填写此表

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

补充剂危害

储存分类代码

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Direct isolation of poly (A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo (T) capture
Jacobsen N, et al.
Nucleic Acids Research, 32(7), e64-e64 (2004)
Comprehensive PCR-based assay for detection and species identification of human herpesviruses
Johnson G, et al.
Journal of Clinical Microbiology, 38(9), 3274-3279 (2000)
Development of a universal internal positive control
Kavlick MF
Biotechniques, 65(5), 275-280 (2018)
Preservation of nucleic acid integrity in guanidine thiocyanate lysates of whole blood
Kravchenko AV, et al.
Russian Journal of Bioorganic Chemistry, 32(6), 547-551 (2006)
Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity
Hosomi K, et al.
Scientific reports, 7(1), 4339-4339 (2017)

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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