To reconstitute dissolve G6378, Glucose-6-phosphate Dehydrogenase, in water and a dilute buffer. Following reconstitution, it should be refrigerated at 4°C, with stock solutions remaining stable for up to 6 months at this temperature.
G6378
葡萄糖-6-磷酸脱氢酶 来源于面包酵母（酿酒酵母）
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
别名:
6-磷酸葡糖脱氢酶, G-6-P-DH
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100 UNITS
$95.20
250 UNITS
$174.00
500 UNITS
$308.00
1000 UNITS
$611.00
2000 UNITS
$1,040.00
5000 UNITS
$2,130.00
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100 UNITS
$95.20
250 UNITS
$174.00
500 UNITS
$308.00
1000 UNITS
$611.00
2000 UNITS
$1,040.00
5000 UNITS
$2,130.00
About This Item
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类型
Type XV
质量水平
表单
lyophilized powder
比活
200-400 units/mg protein (modified Warburg-Christian)
分子量
128 kDa
纯化方式
crystallization
应用
diagnostic assay manufacturing
运输
dry ice
储存温度
−20°C
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应用
葡萄糖-6-磷酸脱氢酶被用于测试玉米胚乳形成中的酮糖还原酶活性。[3]
生化/生理作用
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步<<<17>>>。
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步。
单位定义
一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下，每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。
外形
冻干粉，基本上无硫酸盐，按干重计含有大约20% 的柠檬酸钠
制备说明
由 G7877 制备
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
Anna Leonov et al.
Oncotarget, 13, 918-943 (2022-08-09)
We propose a hypothesis of a mechanism linking cellular aging to cellular quiescence in chronologically aging budding yeast. Our hypothesis posits that this mechanism integrates four different processes, all of which are initiated after yeast cells cultured in a medium
实验方案
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
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What are the recommended reconstitution and storage procedures for item G6378, Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)?
1 answer-
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What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?
1 answer-
The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.
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What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).
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What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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