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Merck

G5768

Sigma-Aldrich

Gamborg's B-5 基本混合盐

fine powder, suitable for plant cell culture

别名:

B-5盐混合物, Gamborg B-5培养基, 基础盐混合物

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1 L
$22.40
10 L
$102.00

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1 L
$22.40
10 L
$102.00

About This Item

UNSPSC代码:
12352207
NACRES:
NA.72

$22.40


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表单

fine powder

质量水平

技术

cell culture | plant: suitable

应用

agriculture

储存温度

2-8°C

一般描述

由Gamborg等描述的Gamborg′B-5培养基[1]可以完全培养基(G5893)进行购买或通过从Vitamin 1000x(G1019)和Basal Salt混合物(G5768)等化学品储备物进行重悬而得。 使用Gamborg′B-5(B5)培养基用于植物细胞系和组织的体外培养,如墨西哥红豆杉、大壶藓、白亚麻、赤桉。

应用

Gamborg′s B-5基础盐混合物已被用于:
  • 制备TEX缓冲液的组分[2]
  • 作为培养基维持马铃薯悬浮及愈伤组织培养[3]
  • 作为主茎节变形方法的培养基[4]

Gamborg′B-5培养基已被用于培养番荔枝愈伤组织。

公式变量

如Gamborg等(1968)所描述的,含有大量和微量营养素的浓度。

培养基配方

数量

配制成每升培养基含 3.1 克粉末。

象形图

Flame over circle

警示用语:

Warning

危险声明

危险分类

Ox. Sol. 3

储存分类代码

5.1B - Oxidizing hazardous materials

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

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Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Ashish Baldi et al.
Biotechnology letters, 30(9), 1671-1677 (2008-04-23)
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and

商品

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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