生物源
mouse
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
JP74, monoclonal
形狀
buffered aqueous solution
物種活性
mouse, rat, human
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction
同型
IgG2a
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... AKAP12(9590)
mouse ... Akap12(83397)
一般說明
Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain.
特異性
Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).
免疫原
fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).
應用
Anti-Gravin antibody has been used in western blotting immunofluorescence.
Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.
生化／生理作用
Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients .
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Jeffrey W Streb et al.
The Journal of biological chemistry, 279(53), 56014-56023 (2004-10-22)
A Kinase Anchoring Protein 12 (AKAP12; also known as src-suppressed C kinase substrate (SSeCKS) and Gravin) is a multivalent anchoring protein with tumor suppressor activity. Although expression of AKAP12 has been examined in a number of contexts, its expression control
Gravin regulates centrosome function through PLK1
Colicino E G, et al.
Molecular Biology of the Cell, 29(5), 532-541 (2018)
Irwin H Gelman
Frontiers in bioscience : a journal and virtual library, 7, d1782-d1797 (2002-07-23)
Scaffolding proteins are thought to facilitate the efficiency and specificity of enzyme/substrate reactions by coordinating their interaction along a cytoskeletal infrastructure in a spacial and temporal manner. Rodent SSeCKS, and its human orthologue, Gravin, seem to function as tumor suppressors
Sookkasem Khositseth et al.
Molecular & cellular proteomics : MCP, 10(1), M110-M110 (2010-10-14)
Previous studies in yeast have supported the view that post-transcriptional regulation of protein abundances may be more important than previously believed. Here we ask the question: "In a physiological regulatory process (the response of mammalian kidney cells to the hormone
Jacob Wang et al.
Journal of gastroenterology, 44(9), 897-911 (2009-05-27)
As the premalignant lesion of human esophageal adenocarcinoma (EAC), Barrett's esophagus (BE) is characterized by intestinal metaplasia in the normal esophagus (NE). Gene expression profiling with microarray and serial analysis of gene expression (SAGE) may help us understand the potential
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