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一般說明
来源于米尔伊丽莎白菌的糖苷内切酶F1是一种聚糖特异性酶。
應用
来源于米尔伊丽莎白菌的糖苷内切酶F1已被用于解除人血色素沉着蛋白 (HFE) 与阳离子非依赖性甘露糖-6-磷酸受体 (CI-MPR) 的结合并用于岩藻糖基化 N-糖肽的糖基化。
切割天冬酰胺连接或游离的低聚甘露糖、以及杂化但不是复合的低聚糖。
生化/生理作用
来源于米尔伊丽莎白菌的糖苷内切酶F1在蛋白质聚糖结构中壳二糖核心的两个N-乙酰葡糖胺残基的裂解中起到重要作用。它介导高甘露糖和杂交寡聚糖的裂解。
包裝
提供5×反应缓冲液,250mM NaH2PO4,pH 5.5。
單位定義
在37℃,pH5.5条件下,一个单位可在每分钟内从1 μmole变性核糖核酸酶B中释放N-连接的寡糖。
外觀
在20mM Tris-HCl(pH 7.5)中无菌填充的溶液
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
其他客户在看
Receptor activity-modifying protein 1 determines the species selectivity of non-peptide CGRP receptor antagonists
The Journal of Biological Chemistry, 277, 14294-14298 (2002)
EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and alpha-acid glycoprotein
BioChemistry: An Indian Journal, 455, 107-118 (2013)
Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM
Journal of proteomics, 189, 67-74 (2018)
In vitro binding of HFE to the cation-independent mannose-6 phosphate receptor
Blood Cells, Molecules and Diseases, 43(2), 180-193 (2009)
FEMS microbiology letters, 325(2), 123-129 (2011-11-19)
It has been demonstrated previously that Enterococcus faecalis produces secreted endoglycosidases that enable the bacteria to remove N-linked glycans from glycoproteins. One enzyme potentially responsible for this activity is EF0114, comprising a typical GH18 endoglycosidase domain and a GH20 domain.
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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