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生物源
Porcine liver
形狀
ammonium sulfate suspension
比活性
≥150 units/mg protein (biuret)
分子量
168 kDa
儲存溫度
2-8°C
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一般說明
酯酶,又称羧基酯水解酶,是水解酶超家族中的一员。
應用
从猪肝中提取的酯酶已用于揭示紫外可见光谱中的荧光。
该酶(来自 Sigma)已被用于开发一种基于凝胶的替代实验方案,用于水性介质中的珠结合催化活性的筛选。该产品还用于研究化合物 L-缬氨酸-3- {8- [(E)-2- [3-甲氧基苯基]乙烯基] -7-甲基-1-炔丙基黄嘌呤-3-基}丙基酯盐酸盐的性质(MSX-4,一种腺苷 A2A 受体拮抗剂 MSX-2 的氨基酸酯前药)。
猪肝酯酶被用于制药和工业应用中以催化五乙酰儿茶素和表儿茶素的水解。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成。
生化/生理作用
猪肝酯酶催化酯的对映选择性转化,产生羧酸。分子量为 168kDa。它是一种丝氨酸酶,每个分子上有两个活性位点,它在稀酸或浓盐存在下解离成活性半分子。
细菌和真菌的酯酶具有多种工业应用。它能催化酯键的水解和合成。
酯酶作用于含有短链脂肪酸的水溶性羧酸酯。其功能来自于Ser、His和Asp/Glu的催化三联体。
單位定義
一个酶活性单位是指在pH 8.0、25℃条件下每分钟将1.0 μM丁酸乙酯水解成丁酸和乙醇所需的酶量。
外觀
悬浮于 3.2M(NH4)2SO4 溶液,pH 8
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
实验方案
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
在测定酯酶活性时,使用丁酸乙酯通过pH滴定速率法进行测定。酯酶可以将酯水解成酸和醇。
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