生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
分子量
antigen ~185 kDa
种属反应性
mouse (predicted), human, rat (predicted), chicken (predicted)
技术
western blot: 1:1,000 using human epidermoid carcinoma A431 cells
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)
一般描述
The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Tyrosine 992 of EGF R is autophosphorylation site and required for increasing the signalling capacity of EGF R
Anti-phospho-EGFR [pTyr992] specifically recognizes human EGFR phosphorylated at tyrosine 992 (~185 kDa). Cross reactivity may be observed with mouse, rat and chicken.
Anti-phospho-EGFR [pTyr992] specifically recognizes human EGFR phosphorylated at tyrosine 992 (~185 kDa). Cross reactivity may be observed with mouse, rat and chicken.
免疫原
synthetic phosphopeptide derived from the region of human EGFR that contains tyrosine 992. The sequence is conserved in mouse and rat with 100% homology. Chicken EGFR is 80% homologous.
应用
Anti-phospho-EGFR [pTyr992] antibody may be used at a working dilution of 1:1000 for immunoblotting using A431 cells.
外形
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+) containing 50% glycerol with 1 mg/mL BSA (protease and IgG-free) and 0.05% sodium azide.
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储存分类代码
10 - Combustible liquids
Nicole K Nickerson et al.
Oncology research, 20(7), 303-317 (2013-07-25)
Epidermal growth factor receptor (EGFR) expression has been linked to progression of basal breast cancers. Many breast cancer cells harbor the EGFR and produce its family of ligands, suggesting they may participate in autocrine and paracrine signaling with cells of
H Keilhack et al.
The Journal of biological chemistry, 273(38), 24839-24846 (1998-09-12)
The protein-tyrosine phosphatase SHP-1 binds to and dephosphorylates the epidermal growth factor receptor (EGFR), and both SH2 domains of SHP-1 are important for this interaction (Tenev, T., Keilhack, H., Tomic, S., Stoyanov, B., Stein-Gerlach, M., Lammers, R., Krivtsov, A. V.
Parthasarathy Seshacharyulu et al.
Expert opinion on therapeutic targets, 16(1), 15-31 (2012-01-14)
Cancer is a devastating disease; however, several therapeutic advances have recently been made, wherein EGFR and its family members have emerged as useful biomarkers and therapeutic targets. EGFR, a transmembrane glycoprotein is a member of the ERBB receptor tyrosine kinase
Rachana Patel et al.
Current pharmaceutical design, 18(19), 2672-2679 (2012-03-07)
Members of epidermal growth factor receptor (EGFR) or ErbB receptor family play a critical role in a wide range of human cancers. In the past decade, there has been a remarkable progress in developing ErbB targeted therapeutics. However, a substantial
A Wells
The international journal of biochemistry & cell biology, 31(6), 637-643 (1999-07-15)
The receptor for the epidermal growth factor (EGF) and related ligands (EGFR), the prototypal member of the superfamily of receptors with intrinsic tyrosine kinase activity, is widely expressed on many cell types, including epithelial and mesenchymal lineages. Upon activation by
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