This product may be swelled in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
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一般說明
来自小牛胸腺DNA的脱氧核醣核酸−纤维素双链，也称为ctDNA，是一种DNA天然来源，专为DNA结合剂研究而设计。ctDNA检出限在2.0ng左右，光散射强度（I(LS)）的线性范围为0.05-1.5 mµg。[1]
應用
来自小牛胸腺DNA的脱氧核醣核酸−纤维素双链用于研究多种蛋白质、核苷酸和化学试剂（包括抗癌剂）的结合参数和结合特异性。 它经常用于下拉试验和色谱分析程序。
品質
可能含有微量的 Tris 和 EDTA。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Lutz Wobbe et al.
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The molecular function of mTERFs (mitochondrial transcription termination factors) has so far only been described for metazoan members of the protein family and in animals they control mitochondrial replication, transcription and translation. Cells of photosynthetic eukaryotes harbour chloroplasts and mitochondria
Yi Zhou et al.
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CD74 mediates MHC class-II antigenic peptide loading and presentation and plays an important role in the pathogenesis of autoimmune diseases, including systemic lupus erythematosus. C57BL/6
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
Liyun Zhang et al.
Scientific reports, 4, 3776-3776 (2014-01-21)
The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and
D Pisetsky et al.
The Journal of rheumatology, 26(9), 1934-1938 (1999-09-24)
To elucidate the epitope structure to DNA by identifying antigenic determinants on bacterial DNA bound by anti-DNA antibodies from normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE). Sera from NHS and patients with SLE were tested by
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Do you have recommendations for how to swell the DNA-cellulose and prepare it for subsequent use with DNA-binding proteins?
1 answer-
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How to dissolve this product to coat elisa plate, please?
1 answer-
This product is not tested for the suitability with coating ELISA plates. It may be helpful to read through the peer-reviewed articles associated with this product: https://www.sigmaaldrich.com/search/d8515?focus=papers&page=1&perpage=30&sort=relevance&term=d8515&type=citation_search
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Can Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515, be exposed to high salt?
1 answer-
DNA-Cellulose is stable in up to 5M NaCl. DNA-Cellulose should not be exposed to solutions of NaOH, since NaOH denatures DNA from double-stranded to single-stranded.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What buffer do you use to swell the Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515?
1 answer-
DNA-Cellulose can be swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
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Can Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA, be used for purification of DNA-dependent RNA Polymerase?
1 answer-
We have not tested Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA for this application. However, the following references utilizes double-stranded DNA cellulose for purification of DNA-dependent RNA Polymerase:J. of Bacteriology, 172, 5624-5630 (1990)Biochem. J.,133, 201-203 (1973)
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How do you regenerate the column using Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515?
1 answer-
A suggested procedure for the regeneration of a DNA-Cellulose column would be to use 5 - 10 column volumes of 2M NaCl in buffer, followed by the same volume of buffer alone.
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How do you store Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515, once it is swollen?
1 answer-
DNA-Cellulose can be stored swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.5 - 8) for several months at 4°C (without a bacteriostat).
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