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Merck

D5523

Sigma-Aldrich

杜氏改良 Eagle 培养基-低葡萄糖

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

杜氏改良Eagle培养基(DMEM)是对Eagle基础培养基 (BME)的改良,含有四倍浓度的氨基酸和维生素以及其他补充组分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。4,500 mg/L葡萄糖的修饰对培养某些类型的细胞效果最佳。

應用

杜氏改良Eagle低葡萄糖培养基已用于人和小鼠间充质干细胞(MSC)的分化和培养。

數量

配制成每升培养基含 10.0 克粉末。

重構

补充 3.7 g/L 碳酸氢钠。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Daniela Steffens et al.
Journal of biomaterials applications, 32(5), 663-676 (2017-10-11)
Laminin-functionalized poly-d,l-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were implanted. The analysis is comprised of the visual aspect and mean
Akon Higuchi et al.
Scientific reports, 5, 10217-10217 (2015-05-15)
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs
Gayatri Jamwal et al.
Biochimica et biophysica acta, 1865(6), 920-931 (2018-04-06)
IGF1R is a ubiquitous receptor tyrosine kinase that plays critical roles in cell proliferation, growth and survival. Clinical studies have demonstrated upregulation of IGF1R mediated signaling in a number of malignancies including colon, breast, and lung cancers. Overexpression of the
Zikai Feng et al.
Pharmaceuticals (Basel, Switzerland), 13(2) (2020-02-16)
Short-chain quinones (SCQs) have been identified as potential drug candidates against mitochondrial dysfunction, which is largely dependent on their reversible redox characteristics of the active quinone core. We recently synthesized a SCQ library of > 148 naphthoquinone derivatives and identified
Kallyne K Mimura et al.
Molecular vision, 18, 1583-1593 (2012-06-29)
The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. C57BL/6 female mice were infected using intravitreal injections of either 10(6) tachyzoites of

实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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